SubtiBank SubtiBank
pcrA

pcrA

ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
locus
BSU_06610
pI
5.77
mw
83.37 kDa
protein length
739 aa Sequence Blast
gene length
2220 bp Sequence Blast
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
product
ATP-dependent DNA helicase
essential
yes
synonyms
yerF

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.2|Additional genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    719,370 → 721,589

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
  • dismantles [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] filaments [Pubmed|20723756]
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
  • Protein family

  • [SW|helicase family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|YjcD]
  • [SW|Domains]

  • two RecA-like domains [Pubmed|24147116]
  • intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
  • [SW|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)
  • [SW|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)
  • Effectors of protein activity

  • unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]
  • helicase activity is stimulated by interaction with [SW|RNA polymerase] [Pubmed|24147116]
  • Structure

  • [PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
  • [PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
  • [PDB|5DMA], C-terminal [SW|RNA polymerase] interaction domain [pubmed|28160601]
  • Expression and Regulation

    Operons

    genes
    [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
    description
    [Pubmed|9701819]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
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    Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 19943906,29856930
  • Original Publications

  • 12073041,9701819,16267290,19943900,20723756,18276648,17449621,10899133,10199404,23326247,24147116,21710567,26070154,28160601