mobA

molybdopterin-guanine dinucleotide biosynthesis

locus: BSU_14260

pI: 6.17

mw: 22.31 kDa

protein length: 199 aa

gene length: 600 bp

function: nitrate respiration

product: molybdenum cofactor synthesis protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.10|Biosynthesis of molybdopterin]

Gene

Coordinates: 1,495,505 → 1,496,104

The protein

Catalyzed reaction/ biological activity

• GTP + H⁺ + Mo-molybdopterin --> diphosphate + Mo-molybdopterin guanine dinucleotide (according to UniProt)

Protein family

• mobA family (single member, according to UniProt)

Structure

• [PDB|1FR9] (from E. coli, 27% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C6DB8499752ED1DBF276C0E46CC561450BED6A40|mobA]-[gene|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB]-[gene|03DBD906E31D6A8A4D14C9E2DACF0F1AB9F8DAA8|moeA]-[gene|A283C34375EDF495940E2F03C4FB6A9FE841FA1E|mobB]-[gene|12F27FA4DB8232B52271565C4F4937520A4F5C7B|moaE]-[gene|5C3EAEE1287FFB1541A31B31A8B6627E60A4D2EB|moaD]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE14260 (Δ[gene|C6DB8499752ED1DBF276C0E46CC561450BED6A40|mobA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATCTCACCTCTAGT, downstream forward: _UP4_TGACGGGGCAATAACATGAT
• BKK14260 (Δ[gene|C6DB8499752ED1DBF276C0E46CC561450BED6A40|mobA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATCTCACCTCTAGT, downstream forward: _UP4_TGACGGGGCAATAACATGAT

References

Reviews

• 23539623

Original publications

• 22383849