malA

6-phospho-alpha-glucosidase

Locus: BSU_08180

Isoelectric point: 4.74

Molecular weight: 50.35 kDa

Protein length: 449 aa

Gene length: 1350 bp

Function: maltose utilization

Product: 6-phospho-alpha-glucosidase

Essential: no

E.C.: 3.2.1.122

Synonyms: glvG,glvA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of maltose
• Groups of genes → Membrane proteins

Gene

Coordinates: 890,022 → 891,371

The protein

Catalyzed reaction/ biological activity

• α-maltose 6'-phosphate + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• Glycosyl hydrolase 4 family (according to UniProt)

Structure

• 1U8X

Localization

• membrane associated PubMed

Expression and Regulation

Operons

• Genes: malA-glvR-malP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GlvR: activation, PubMed, in GlvR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induction by maltose (GlvR) PubMed

Biological materials

Mutant

• MGNA-C289 (glvA::erm), available at the NBRP B. subtilis, Japan
• BKE08180 (ΔmalA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT
• BKK08180 (ΔmalA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT

References

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