desK [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

two-component sensor kinase, regulation of cold shock expression of des

locus: BSU19190

pI: 9.00

mw: 42.00 kDa

function: regulation of cold shock expression of des

product: two-component sensor kinase

essential: no

synonyms: yocF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

[SW|Categories] containing this gene/protein

• [SW|lipid metabolism/ other], [SW|protein modification], [SW|transcription factors and their control], [SW|cold stress proteins], [SW|membrane proteins], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 2,090,574 -> 2,091,686

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|search|DesR]

[SW|Domains]

• 5 transmembrane helices
• cytoplasmatic C-terminal trail

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• unsaturated fatty acids are negative effectors of the system

Structure

• [PDB|3EHF]

[SW|Localization]

• membrane (transmembrane segments)

[SW|Interactions]

• [protein|search|DesK]-[protein|search|DesR]

Additional information

• [protein|search|DesK] has the ability to sense changes in membrane fluidity [Pubmed|17087771]

Expression and Regulation

Operon

• ''[protein|search|desK]-[protein|search|desR]'' [Pubmed|11285232]

Regulation

• induced by cold shock (12-fold) [Pubmed|12399512]

Biological materials

Mutant

• MGNA-A327 (yocF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/327 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
• [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 20117042,17087771,24819366

Original publications

• 10094672,14734164,12399512,20507988,19233289,11285232,19805278,15090506,12207704,20705470,23356219,24574048,24522108,25406381,26172072,27528507