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mstX
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mstX

membrane-integrating protein for membrane protein expression, MISTIC
locus
BSU_31321
pI
4.00
mw
13.00 kDa
protein length
110 aa Sequence Blast
gene length
333 bp Sequence Blast
function
unknown
product
MISTIC
essential
no
synonyms
Outlinks
KEGGBsubCycUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,218,525 → 3,218,857

    Phenotypes of a mutant

  • reduced [SW|biofilm formation] (can be suppressed by inactivation of ''[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]'') [Pubmed|23737939]
  • overexpression result in increased [SW|biofilm formation] even in a domesticated strain background [Pubmed|23737939]

    • The protein

    Structure

  • [PDB|1YGM] [Pubmed|15731457]

    • [SW|Localization]

  • integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery [Pubmed|15731457]

    • Expression and Regulation

    Operons

    genes
    [gene|C5C72925567927CC177CCAC5369F80D57B9FAB5F|mstX]-[gene|752860E73AD1A72D6598F995B80F5B21A77A5CBD|yugO]
    description
    [Pubmed|23737939]

    regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|23737939], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

    • regulation

  • repressed by [protein|search|SinR] [Pubmed|23737939]

    • additional information

  • potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain [Pubmed|26503040]
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    Biological materials

    Mutant

  • BKE31321 (Δ[gene|C5C72925567927CC177CCAC5369F80D57B9FAB5F|mstX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31321 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACCGGATTTCTC, downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA
  • BKK31321 (Δ[gene|C5C72925567927CC177CCAC5369F80D57B9FAB5F|mstX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31321 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAACCGGATTTCTC, downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA

    • References

  • 16704729,19475664,21097953,15731457,23737939,25177765,31459283