SubtiBank SubtiBank
trmK
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trmK

tRNA:m1A22 methyl transferase
locus
BSU25180
pI
5.27
mw
23.56 kDa
protein length
216 aa Sequence Blast
gene length
651 bp Sequence Blast
function
tRNA modification
product
tRNA:m1A22 methyl transferase
essential
no
synonyms
yqfN

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    2,598,616 → 2,599,266

    The protein

    Catalyzed reaction/ biological activity

  • Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]
  • adenosine22 in tRNA + S-adenosyl-L-methionine --> H+ + N1-methyladenosine22 in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]
  • Protein family

  • TrmK family (according to UniProt)
  • [SW|Domains]

  • N-terminal Class I MTase domain [pubmed|30931478]
  • C-terminal coiled-coil domain [pubmed|30931478]
  • Modification

  • phosphorylation on Ser-48 [Pubmed|17218307]
  • [SW|Cofactors]

  • SAM [pubmed|30931478]
  • Structure

  • [PDB|6Q56] [pubmed|30931478]
  • [SW|Localization]

  • cytoplasm (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]

    description

  • [pubmed|22383849]
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    Biological materials

    Mutant

  • BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
  • BKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
  • References

  • 18420655,17218307,27098549,30931478