hepS [2019-07-04 12:49:53]

heptaprenyl diphosphate synthase component I

locus: BSU22760

pI: 6.27

mw: 28.97 kDa

protein length: 251 aa

gene length: 756 bp

function: menaquinone biosynthesis

product: heptaprenyl diphosphate synthase component I (together with [protein|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|HepT])

essential: no

ec: 2.5.1.30

synonyms: gerCA,hepA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]

Gene

Coordinates: 2,383,615 → 2,384,370

Phenotypes of a mutant

• essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• All-trans-hexaprenyl diphosphate + isopentenyl diphosphate = diphosphate + all-trans-heptaprenyl diphosphate (according to Swiss-Prot)

Protein family

• Methylisocitrate lyase family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE22760 (Δ[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATATCACCCTTGTCC, downstream forward: _UP4_TAAACCATTATGCAGGACTC
• BKK22760 (Δ[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATATCACCCTTGTCC, downstream forward: _UP4_TAAACCATTATGCAGGACTC

References

• 23840410,28189581