nth [2017-8-11 16:0:3]

apurinic/apyrimidinic endonuclease , endonuclease III, removes abasic sites during base excision repair

locus: BSU22340

pI: 9.28

mw: 24.85 kDa

protein length: 219 aa

gene length: 657 bp

function: DNA repair

product: endonuclease III

essential: no

synonyms: jooB, ypoB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]

Gene

Coordinates: 2,344,755 → 2,345,414

Phenotypes of a mutant

• the mutant is sensititive to exposure to H2O2 [Pubmed|21954439]

The protein

Catalyzed reaction/ biological activity

• The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)

Effectors of protein activity

• activity is stimulated by untwisting of the DNA (by [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|DnaD], [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs], or [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]) [Pubmed|21954439]

Structure

• [PDB|1P59] (complex with DNA, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
  description: [Pubmed|22383849]

• genes: [gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]
  description: [Pubmed|21954439]

  regulation

  • constitutively expressed [Pubmed|21954439]

References

Reviews

• 22933559

Original publications

• 12840008,21954439,24914186