minJ

topological determinant of [SW|cell division], part of the Min system (with Z ring placement)

locus: BSU_35220

pI: 9.06

mw: 43.51 kDa

protein length: 397 aa

gene length: 1194 bp

function: [SW|cell division] site selection

product: topological determinant of [SW|cell division]

essential: no

synonyms: yvjD,swrAB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.1|The Min system]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,620,346 → 3,621,539

Phenotypes of a mutant

• inactivation of [gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ] reduces sporulation efficiency to 36% that of wild type cells; delayed entry into sporulation and aberrant forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle [Pubmed|20352045]
• required for oriC placement during spore development [Pubmed|27059541]

Protein family

• MinJ family (single member, according to UniProt)

[SW|Domains]

• 7 transmembrane helices (aa 15- 269) (according to UniProt)
• [SW|PDZ domain] (aa 295 - 361) (according to UniProt)

[SW|Localization]

• forms rings at the division septum [Pubmed|22108385]
• cell membrane [Pubmed|23264578]

Expression and Regulation

Operons

• genes: [gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]-[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
  description: [Pubmed|16030230]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18567663], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|18567663], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, ([protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA] promoter) [Pubmed|18567663], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

• genes: [gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
  description: [pubmed|18976281]

Biological materials

Mutant

• MGNA-B648 (minJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1647 NBRP B. subtilis, Japan]
• BKE35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA, downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG
• BKK35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA, downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]
• [SW|Marc Bramkamp], University of Cologne, Cologne, Germany [http://www.kraemerlab.uni-koeln.de/celldivision.php homepage]

References

Reviews

• 19884039,24367361,28500523

Original Publications

• 19019154,16030230,18976281,20352045,18567663,22108385,22457634,23264578,26735940,27059541,28674273,29403445