SubtiBank SubtiBank
iolE
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iolE

2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
locus
BSU_39720
pI
5.21
mw
33.44 kDa
protein length
297 aa Sequence Blast
gene length
894 bp Sequence Blast
function
myo-inositol catabolism
product
2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
essential
no
ec
4.2.1.44
synonyms
yxdE

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • Gene

    Coordinates
    4,078,173 → 4,079,066

    The protein

    Catalyzed reaction/ biological activity

  • scyllo-inosose --> 3D-3,5/4-trihydroxycyclohexane-1,2-dione + H2O (according to UniProt)
  • Protein family

  • IolE/MocC family (single member, according to UniProt)
  • Structure

  • [PDB|3CNY] (from ''Lactobacillus plantarum wcfs1 mutant'', 63% identity, 78% similarity)
  • Expression and Regulation

    Operons

    genes
    [gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
    description
    [Pubmed|9226270]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9226270], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]: repression, [Pubmed|9887260,9226270], in [regulon|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • regulation

  • induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]
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    Biological materials

    Mutant

  • MGNA-B774 (iolE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1773 NBRP B. subtilis, Japan]
  • BKE39720 (Δ[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
  • BKK39720 (Δ[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
  • labs

  • [SW|Yasutaro Fujita], University of Fukuyama, Japan
  • [[Ken-ichi Yoshida]], Kobe University, Japan
  • References

  • 9226270,14993306,9887260,18310071,14993306