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gpsB [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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gpsB [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

required for removal of PBP1 from the cell pole after completion of cell pole maturation
locus
BSU22180
pI
5.00
mw
11.00 kDa
function
cell division, cell elongation
product
cell division protein, member of the divisome
essential
no
synonyms
ypsB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|cell division], [SW|membrane proteins], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,331,779 -> 2,332,075

    Phenotypes of a mutant

  • synthetic lethal phenotype with a ''[protein|search|ftsA]'' mutation [Pubmed|18776011]; synthetic sick phenotype when combined with an ''[protein|search|ezrA]'' mutation [Pubmed|18363795], although this seems controversial [Pubmed|18776011]
  • reduced growth at high salt [Pubmed|25845974]

    • The protein

    Catalyzed reaction/ biological activity

  • activates [protein|search|PrkC] [Pubmed|25845974]

    • Paralogous protein(s)

  • [SW|DivIVA]

    • [SW|Domains]

  • contains a [SW|DivIVA]-like N-terminal domain

    • Modification

  • phosphorylation on Thr-75 by [protein|search|PrkC] [Pubmed|25845974,17218307]

    • Structure

  • GpsB is hexameric (trimer of dimers) [Pubmed|26575090]
  • [PDB|4UG3] (N-terminal domain, aa 1-68) [Pubmed|26575090]
  • [PDB|5AN5] (C-terminal domain, aa 76-98) [Pubmed|26575090]

    • [SW|Localization]

  • dynamic, shuttles between lateral membrane and the divison septum [Pubmed|18363795], localizes to the divisome after Z-ring formation [Pubmed|18776011]

    • [SW|Interactions]

  • [protein|search|GpsB]-[protein|search|GpsB] [Pubmed|18363795, 26575090]
  • [protein|search|GpsB]-[protein|search|EzrA] [Pubmed|18363795]
  • [protein|search|GpsB]-[protein|search|PonA] [Pubmed|18363795] via GpsB N-terminal domain and cytosolic N-terminus of PBP1 [Pubmed|26575090]
  • [protein|search|GpsB]-[protein|search|MreC] [Pubmed|18363795]
  • [protein|search|GpsB]-[protein|search|PrkC] [Pubmed|25845974]

    • Biological materials

    Mutant

  • 1A934 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
  • 1A970 ( ''gpsB''::''kan''), [Pubmed|18776011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]

    • GFP fusion

  • [Pubmed|18363795, 18776011]

    • two-hybrid system

  • [Pubmed|18363795]

    • Antibody

  • raised against L. monocytogenes GpsB [Pubmed|26575090] available in [SW|Sven Halbedel] 's lab

    • References

  • 18776011,25845974,19429628,17218307,18363795,26575090,27257764