SubtiBank SubtiBank
gpsB
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gpsB

cell cycle regulator, cytosolic adaptor for multiple cell wall enzymes, required for removal of PBP1 from the cell pole after completion of cell pole maturation
Locus
BSU_22180
Isoelectric point
5.63
Molecular weight
11.45 kDa
Protein length
Gene length
297 bp Sequence Blast
Function
cell division, cell elongation, spatiotemporal control of penicillin‐binding protein activity
Product
cell division adaptor protein, member of the divisome
Essential
no
Synonyms
ypsB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,331,779 → 2,332,075

Phenotypes of a mutant

  • synthetic lethal phenotype with a ftsA mutation PubMed; synthetic sick phenotype when combined with an ezrA mutation PubMed, although this seems controversial PubMed
  • reduced growth at high salt PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • activates PrkC PubMed
  • Protein family

  • GpsB family (single member, according to UniProt)
  • Paralogous protein(s)

    Domains

  • contains a DivIVA-like N-terminal lipid binding domain PubMed
  • Modification

  • phosphorylation on Thr-75 by PrkC PubMed
  • Structure

  • 4UG3 (N-terminal domain, aa 1-68) PubMed
  • 5AN5 (C-terminal domain, aa 76-98) PubMed
  • GpsB is hexameric (trimer of dimers) PubMed
  • 6GP7 (Complexed with peptide fragment of PBP1A) PubMed
  • 6GPZ (Complexed with peptide fragment of L. monocytogenes PBP1A) PubMed
  • Localization

  • dynamic, shuttles between lateral membrane and the divison septum PubMed, localizes to the divisome after Z-ring formation PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-A472 (gpsB::erm), available at the NBRP B. subtilis, Japan
  • 1A934 ( gpsB::kan), PubMed, available at BGSC
  • 1A970 ( gpsB::kan), PubMed, available at BGSC
  • BKE22180 (ΔgpsB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT
  • BKK22180 (ΔgpsB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT
  • GFP fusion

    Two-hybrid system

    Antibody

  • raised against L. monocytogenes GpsB PubMed available in Sven Halbedel 's lab
  • References

    Reviews

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    Original Publications

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