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gpsB
cell cycle regulator, cytosolic adaptor for multiple cell wall enzymes, required for removal of
PBP1 from the cell pole after completion of cell pole maturation
Molecular weight
11.45 kDa
Function
cell division, cell elongation, spatiotemporal control of penicillin‐binding protein activity
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,331,779 → 2,332,075
Phenotypes of a mutant
synthetic lethal phenotype with a ftsA mutation PubMed; synthetic sick phenotype when combined with an ezrA mutation PubMed, although this seems controversial PubMedreduced growth at high salt PubMed The protein
Catalyzed reaction/ biological activity
Protein family
GpsB family (single member, according to UniProt)Paralogous protein(s)
Domains
contains a DivIVA-like N-terminal lipid binding domain PubMed Modification
Structure
4UG3 (N-terminal domain, aa 1-68) PubMed5AN5 (C-terminal domain, aa 76-98) PubMedGpsB is hexameric (trimer of dimers) PubMed6GP7 (Complexed with peptide fragment of PBP1A) PubMed6GPZ (Complexed with peptide fragment of L. monocytogenes PBP1A) PubMed Localization
dynamic, shuttles between lateral membrane and the divison septum PubMed, localizes to the divisome after Z-ring formation PubMed Expression and Regulation
Biological materials
Mutant
MGNA-A472 (gpsB::erm), available at the NBRP B. subtilis, Japan1A934 ( gpsB::kan), PubMed, available at BGSC1A970 ( gpsB::kan), PubMed, available at BGSCBKE22180 (ΔgpsB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAATBKK22180 (ΔgpsB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCACCTCGTATC, downstream forward: _UP4_TGATCACGCTTGAAAAAAAT GFP fusion
Two-hybrid system
Antibody
References
Reviews
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Original Publications
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