SubtiBank SubtiBank
yclQ [2017-10-15 16:36:57]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yclQ [2017-10-15 16:36:57]

petrobactin (3.4-catecholate siderophore) ABC transporter (binding protein), major component of the secretome
locus
BSU03830
pI
6.63
mw
34.64 kDa
protein length
317 aa Sequence Blast
gene length
951 bp Sequence Blast
function
acquisition of iron
product
petrobactin ABC transporter (binding protein)
essential
no
synonyms
fpiA
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    435,036 → 435,989

    The protein

    Catalyzed reaction/ biological activity

  • uptake of the siderophore petrobactin [Pubmed|19955416]

    • Protein family

  • Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

    • Structure

  • [PDB|3GFV]

    • [SW|Localization]

  • associated to the membrane (via [protein|552D4C42DCEA4625000160D2625711C61AB11661|FpbN]-[protein|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|FpbO]) [Pubmed|10092453,18763711]
  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
    description
    [Pubmed|19955416]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C067 (yclQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2065 NBRP B. subtilis, Japan]
  • BKE03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA, downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC
  • BKK03830 (Δ[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA, downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC

    • References

  • 10092453,19955416,12354229,18957862,18763711,26883633