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gapB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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gapB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
Locus
BSU29020
Isoelectric point
6.00
Molecular weight
37.00 kDa
Function
anabolic enzyme in gluconeogenesis
Product
glyceraldehyde-3-phosphate dehydrogenase 2
Essential
no
E.C.
1.2.1.59
Synonyms
ppc
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis

    • Categories containing this gene/protein

  • carbon core metabolism, sporulation/ other

    • This gene is a member of the following regulons

  • CcpN regulon

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,967,032 -> 2,968,054

    Phenotypes of a mutant

  • inactivation of gapB reduces sporulation efficiency to 0.3% that of wild type cells; delayed entry into sporulation and fewer sporulating cells PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate phosphate NAD(P) = 3-phospho-D-glyceroyl phosphate NAD(P)H (according to Swiss-Prot)
  • This reaction is part of the gluconeogenesis

    • Protein family

  • glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • GapA

    • Kinetic information

  • Michaelis-Menten PubMed

    • Domains

  • Nucleotid bindinge domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)

    • Cofactors

  • NADP (preferentially) and NAD PubMed

    • Structure

  • 3PRL (from B. halodurans)

    • Localization

  • Cytoplasm (Homogeneous) PubMed

    • Expression and Regulation

    Operon

  • gapB-speD PubMed

    • Sigma factor

  • SigA PubMed

    • Regulation

  • repressed in the presence of glucose (70-fold) (CcpN) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

    • Regulatory mechanism

  • CcpN: transcription repression PubMed

    • Biological materials

    Mutant

  • GP701 (gapB::spec), available in Stülke lab
  • 1A1004 ( gapB::erm), [Pubmed| ], available at BGSC

    • Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

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