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gapB
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gapB

glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
locus
BSU_29020
pI
6.45
mw
37.32 kDa
protein length
340 aa Sequence Blast
gene length
1023 bp Sequence Blast
function
anabolic enzyme in gluconeogenesis
product
glyceraldehyde-3-phosphate dehydrogenase 2
essential
no
ec
1.2.1.59
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]

    • Gene

    Coordinates
    2,967,032 → 2,968,054

    Phenotypes of a mutant

  • inactivation of ''[gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]'' reduces sporulation efficiency to 0.3% that of wild type cells; delayed entry into sporulation and fewer sporulating cells [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate + NADP+ + phosphate --> 3-phospho-D-glyceroyl phosphate + H+ + NADPH(according to UniProt)
  • D-glyceraldehyde 3-phosphate + NAD+ + phosphate --> 3-phospho-D-glyceroyl phosphate + H+ + NADH (according to UniProt)
  • This reaction is part of the gluconeogenesis

    • Protein family

  • glyceraldehyde-3-phosphate dehydrogenase family (with [protein|EB6512177418B1601C6641FB2DEE99C2CD10E671|GapA], according to UniProt)

    • Paralogous protein(s)

  • [protein|EB6512177418B1601C6641FB2DEE99C2CD10E671|GapA]

    • Kinetic information

  • Michaelis-Menten [Pubmed|10799476]

    • [SW|Domains]

  • Nucleotid bindinge domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)

    • [SW|Cofactors]

  • NADP+ (preferentially) and NAD+ [Pubmed|10799476]

    • Structure

  • [PDB|3PRL] (from ''B. halodurans'')

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • additional information

  • degraded after a shift from malate to glucose with an estimated half-life time of approx. 3 hours by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [pubmed|28760849]

    • Biological materials

    Mutant

  • MGNA-A121 (gapB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/121 NBRP B. subtilis, Japan]
  • GP701 (''gapB''::''spec''), available in [SW|Stülke] lab
  • 1A1004 ( ''gapB''::''erm''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1004&Search=1A1004 BGSC]
  • BKE29020 (Δ[gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGGACACCCCTTATC, downstream forward: _UP4_TAAAATAAGGTCATGGACAC
  • BKK29020 (Δ[gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGGACACCCCTTATC, downstream forward: _UP4_TAAAATAAGGTCATGGACAC

    • labs

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

  • 16479537,10799476,10844697,15720552,18586936,17114254,22190493,23033921,22740702,26735940,28760849