SubtiBank SubtiBank
glnK [2019-08-27 12:46:08]
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glnK [2019-08-27 12:46:08]

two-component sensor kinase, regulation of the [gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]-[gene|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT] operon
locus
BSU_02440
pI
5.73
mw
44.00 kDa
protein length
410 aa Sequence Blast
gene length
1233 bp Sequence Blast
function
regulation of the [gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]-[gene|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT] operon
product
two-component sensor kinase
essential
no
synonyms
ycbA,yzgA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    265,476 → 266,708

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|GlnL]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • [SW|Domains]

  • four transmembrane segments, C-terminal histidine phosphotransferase domain
  • [SW|Histidine kinase] (aa 189-405) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|C18B886D06879C64B64606371E85A7D4D22DCFAC|glnK]-[gene|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • BKE02440 (Δ[gene|C18B886D06879C64B64606371E85A7D4D22DCFAC|glnK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGGCCAGTATATATC, downstream forward: _UP4_ATTCAGAAAGGGTGAACCAA
  • BKK02440 (Δ[gene|C18B886D06879C64B64606371E85A7D4D22DCFAC|glnK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGGCCAGTATATATC, downstream forward: _UP4_ATTCAGAAAGGGTGAACCAA

    • References

  • 10094672,15995196