phrC

response regulator aspartate phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)

locus: BSU_03780

pI: 8.16

mw: 4.06 kDa

protein length: 40 aa

gene length: 123 bp

function: control of ComA activity

product: phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
• [category|SW 6|Groups of genes] → [category|SW 6.8|Short peptides]

Gene

Coordinates: 429,963 → 430,085

The protein

Catalyzed reaction/ biological activity

• binds to [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|RapC], this results in the inability of [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|RapC] to interact with [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] [Pubmed|12950917]

Protein family

• [SW|phr family] (according to UniProt)

Structure

• [PDB|4GYO] (complex [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|RapJ]-[protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|PhrC]) [Pubmed|23526881]

Expression and Regulation

Operons

• genes: [gene|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC]-[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]
  description: [Pubmed|10464187]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10464187], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|10464187], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10464187], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

• genes: [gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]
  description: [Pubmed|10464187]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

Biological materials

Mutant

• BKE03780 (Δ[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTTAGATTTCAATTTCATA, downstream forward: _UP4_TAAGAACAAGCCCCTTCTCA
• BKK03780 (Δ[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTTAGATTTCAATTTCATA, downstream forward: _UP4_TAAGAACAAGCCCCTTCTCA

References

Reviews

• 22024380

Original publications

• 12950917,16816200,19202088,18375560,11267663,10464187,12850135,16091051,23526881,22511326