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tasA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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tasA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

major component of biofilm matrix, forms amyloid fibers
locus
BSU24620
pI
5.00
mw
28.00 kDa
function
biofilm formation
product
major component of biofilm matrix
essential
no
synonyms
cotN, yqhF

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.2|Amyloid protein synthesis, secretion and assembly]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|RemA regulon], [SW|SinR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,553,081 -> 2,553,866

    Phenotypes of a mutant

  • altered cell death pattern in colonies [Pubmed|23012477]
  • The protein

    Catalyzed reaction/ biological activity

  • forms amyloid fibers that bind cells together in the biofilm [Pubmed|20080671]
  • Protein family

  • peptidase M73 family (according to Swiss-Prot)
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862], secretion requires [protein|search|SipW] [Pubmed|10049401]
  • membrane [Pubmed|18763711]
  • [SW|Interactions]

  • forms fibers of variable length and 10-15 nm in width [Pubmed|20080671]
  • [protein|search|TapA]-[protein|search|TasA] [Pubmed|24488317]
  • [protein|search|SipW]-[protein|search|TasA] [Pubmed|10049401]
  • Expression and Regulation

    Operon

  • ''[protein|search|tapA]-[protein|search|sipW]-[protein|search|tasA]'' [Pubmed|16430695]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|16430695]
  • Regulation

  • repressed by casamino acids [Pubmed|12107147]
  • repressed by [protein|search|SinR] [Pubmed|16430695]
  • repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
  • Regulatory mechanism

  • [protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10464223,17720793 PubMed]
  • [protein|search|SinR]: transcription repression [Pubmed|16430695]
  • [protein|search|RemA]: transcription activation [Pubmed|23646920]
  • Additional information

  • induction by sequestration of [protein|search|SinR] by [protein|search|SinI] or [protein|search|SlrA] [PubMed|15661000,19788541] or by SlrR [PubMed|20351052]
  • the '[protein|search|tapA]-[protein|search|sipW]-[protein|search|tasA]' operon is not expressed in a '[protein|search|ymdB]' mutant [PubMed|21856853]
  • the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the '[protein|search|sinR]' mRNA) [PubMed|21815947]
  • References

    Reviews

  • 20735481,20351052,21488983,24988880,25907113,26418850
  • Original publications

  • 23352144,23352134,23632024,23012477,21815947,21856853,18047568,16430695,16430696,10368135,18430133,18647168,10464223,17720793,18957862,12107147,18763711,20080671,10049401,23646920,24488317,25894589