rhgR

transcriptional activator ([SW|AraC family]) of the rhamnogalacturonan operon

locus: BSU_07010

pI: 7.77

mw: 87.56 kDa

protein length: 761 aa

gene length: 2286 bp

function: regulation of pectin utilization

product: transcriptional activator of the rhamnogalacturonan operon

essential: no

synonyms: yesS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 765,838 → 768,123

The protein

Protein family

• [SW|AraC family]

[SW|Domains]

• [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]-binding domain: aa 406 - 510 [Pubmed|19651770]
• [SW|HTH araC/xylS-type domain] (aa 659-756) (according to UniProt)

Effectors of protein activity

• interaction with [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr(His)-P]]] (occurs in the absence of preferred carbon sources) stimulates YesS activity [Pubmed|19651770]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
  description: [Pubmed|19651770]

  regulatory mechanism

  • [protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR]: activation, [Pubmed|19651770], in [regulon|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR regulon]

  regulation

  • induced by pectin ([protein|search|RhgR]) [Pubmed|19651770]

Biological materials

Mutant

• MGNA-B450 (yesS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1449 NBRP B. subtilis, Japan]
• BKE07010 (Δ[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG
• BKK07010 (Δ[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG

References

• 19651770,17449691,21630458,24391637