yocH

peptidoglycan hydrolase (amidase)

locus: BSU_19210

pI: 8.37

mw: 30.03 kDa

protein length: 287 aa

gene length: 864 bp

function: cell wall turnover

product: peptidoglycan hydrolase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,092,899 → 2,093,762

The protein

[SW|Domains]

• contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
• 2 [SW|LysM domain]s (aa 26-69, aa 78-121) (according to UniProt)

Structure

• [PDB|4WLK] ([protein|3DAE76660E0CB213155265A3E8DF78A992883F87|YuiC], corresponds to aa 155 ... 286 of [protein|BEBA71A2EF56DAEAF80924856DA68124805BB158|YocH], 34% identity) [pubmed|26163297]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]
  description: [Pubmed|12950927]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12950927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • expression is modulated in response to D,L-endopeptidase activity (increased upon reduced activity in the absence of [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] or [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE]-[protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|FtsX], decreased upon overexpression of [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE]) ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]) [pubmed|31808740]
  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

• genes: [gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB]-[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]-[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A321 (yocH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/321 NBRP B. subtilis, Japan]
• AH023 knock-out mutant (kan), available in [SW|Kevin Devine]'s and [SW|Jörg Stülke]'s labs [Pubmed|17581128]
• BKE19210 (''[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE19210 (Δ[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATA
• BKK19210 (Δ[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATA

Expression vectors

• pGP1691, expression of ''yocH''-Strep in ''B. subtilis'', in [SW|pGP382], available in [SW|Jörg Stülke]'s lab
• pGP1693, expression of ''yocH''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

labs

• [SW|Kevin Devine]

References

Reviews

• 18430080

Original publications

• 14651647,15101989,18957862,12950927,20059685,20070526,23199363,17581128,31808740,26163297