SubtiBank SubtiBank
rplO
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rplO

ribosomal protein
Locus
BSU_01350
Isoelectric point
10.79
Molecular weight
15.24 kDa
Protein length
146 aa Sequence Blast
Gene length
441 bp Sequence Blast
Function
translation
Product
ribosomal protein L15
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins

    • Gene

    Coordinates
    144,085 → 144,525

    The protein

    Protein family

  • universal ribosomal protein uL15 family (single member, according to UniProt)

    • Structure

  • 3J9W (the ribosome) PubMed

    • Expression and Regulation

    Operons

    Genes
    rplO-secY-adk-map

    Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response

    • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

    • Additional information

  • strongly expressed
    • view in new tab

    Genes
    rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-ybzG-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, in stringent response

    • Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed

    • Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE01350 (ΔrplO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAGGACACCTCCTTATC, downstream forward: _UP4_GGCGGTACAGCTGAGGTGAT
  • BKK01350 (ΔrplO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAGGACACCTCCTTATC, downstream forward: _UP4_GGCGGTACAGCTGAGGTGAT

    • References

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