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cheD [Mon Aug 31 2015 13:54:20 GMT+0200 (CEST)]
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cheD [Mon Aug 31 2015 13:54:20 GMT+0200 (CEST)]

chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
locus
BSU16460
pI
9.35
mw
17.00 kDa
function
motility and chemotaxis
product
protein deaminase, CheC activity modulator
essential
no
ec
3.5.1.44
synonyms
ylxK

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.7|Protein deaminase]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.1|Soluble signalling proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]
  • syns
    ylxK

    [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|motility and chemotaxis]
  • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon], [SW|DegU regulon], [SW|SigD regulon], [SW|Spo0A regulon]
  • Gene

    Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
  • The protein

    Catalyzed reaction/ biological activity

  • binds chemoreceptors and increases their ability to activate the kinase activity of [protein|search|CheA] [Pubmed|23226535]
  • Protein L-glutamine + H2O = protein L-glutamate + NH3 (according to Swiss-Prot)
  • deaminates Gln-586, Gln-593, and Gln594 in [protein|search|McpA] [Pubmed|22931217]
  • deaminates Gln-609 in [protein|search|McpC] [Pubmed|22931217]
  • Protein family

  • cheD family (according to Swiss-Prot)
  • [SW|Interactions]

  • [protein|search|CheC]-[protein|search|CheD] [Pubmed|17908686], this interaction is enhanced by [protein|search|CheY]-P [Pubmed|23226535]
  • [protein|search|CheD]-[protein|search|McpA], deamination of Gln-586, Gln-593 and Gln-594 in [protein|search|McpA] [Pubmed|22931217]
  • [protein|search|CheD]-[protein|search|McpC], deamination of Gln-609 in [protein|search|McpC] [Pubmed|22931217]
  • [protein|search|McpB]-[protein|search|CheD] to increase the ability of [protein|search|McpB] to activate [protein|search|CheA] [Pubmed|25039821]
  • Expression and Regulation

    Operon

  • part of the [[fla-che operon]]
  • ''[protein|search|flgB]-[protein|search|flgC]-[protein|search|fliE]-[protein|search|fliF]-[protein|search|fliG]-[protein|search|fliH]-[protein|search|fliI]-[protein|search|fliJ]-[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|9657996,8157612,15175317]
  • ''[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|20233303]
  • [SW|Sigma factor]

  • ''[protein|search|flgB]'': [protein|search|SigA] [Pubmed|9657996], [protein|search|SigD] [Pubmed|9657996]
  • ''[protein|search|ylxF]'': [protein|search|SigD] [Pubmed|20233303]
  • Regulation

  • see [[fla-che operon]]
  • Regulatory mechanism

  • see [[fla-che operon]]
  • Biological materials

    Mutant

  • 1A861 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]
  • 1A863 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A863&Search=1A863 BGSC]
  • DS6868 (marker-less in NCIB3610) [Pubmed|25313396]
  • References

    Reviews

  • 18774298,18990184
  • Original publications

  • 25313396,26122431,17850253,21515776,22931217,23226535,7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647,9657996,8157612,15175317,16469702,24386445,25039821