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cheD [2019-04-16 12:07:17]
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cheD [2019-04-16 12:07:17]

chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
Locus
BSU16460
Isoelectric point
9.35
Molecular weight
17.86 kDa
Protein length
166 aa Sequence Blast
Gene length
501 bp Sequence Blast
Function
motility and chemotaxis
Product
protein deaminase, CheC activity modulator
Essential
no
E.C.
3.5.1.44
Synonyms
ylxK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein deaminase
  • Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble signalling proteins
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for efficient pellicle biofilm formation

    • Gene

    Coordinates
    1,715,970 → 1,716,470

    Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • binds chemoreceptors and increases their ability to activate the kinase activity of CheA PubMed
  • Protein L-glutamine + H2O = protein L-glutamate + NH3 (according to Swiss-Prot)
  • deaminates Gln-586, Gln-593, and Gln594 in McpA PubMed
  • deaminates Gln-609 in McpC PubMed

    • Protein family

  • cheD family (according to Swiss-Prot)

    • Structure

  • 2F9Z (from Thermotoga maritima, 41% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

    • Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • CodY: repression, in CodY regulon
  • DegU: regulation, in DegU regulon
  • SwrA: activation, in SwrA regulon

    • Regulation

  • see fla-che operon
    • view in new tab

    Genes
    flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

    • Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

    • Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
    • view in new tab

    Biological materials

    Mutant

  • 1A861 ( cheD::cat), PubMed, available at BGSC
  • 1A863 ( cheD::cat), PubMed, available at BGSC
  • DS6868 (marker-less in NCIB3610) PubMed
  • BKE16460 (ΔcheD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATAACAACAGCCTCAGTTG, downstream forward: _UP4_TAATTAAGGTATTAGGGGGA
  • BKK16460 (ΔcheD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATAACAACAGCCTCAGTTG, downstream forward: _UP4_TAATTAAGGTATTAGGGGGA

    • References

    Reviews

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    Original publications

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