yrvJ

similar to N-acetylmuramoyl-L-alanine amidase

locus: BSU_27580

pI: 5.19

mw: 55.33 kDa

protein length: 518 aa

gene length: 1557 bp

function: cell wall metabolism

product: peptidoglycan hydrolase

essential: no

ec: 3.5.1.28

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.6|Cell wall/ other/ based on similarity]

Gene

Coordinates: 2,818,494 → 2,820,050

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [SW|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

[SW|Domains]

• contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|CwlC], [protein|B09FB626274B81F00A4FB42D188E089095343182|CwlD], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|LytC], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|YqiI])
• [SW|MurNAc-LAA domain] (aa 346-514) (according to UniProt)
• 4 [SW|SH3b domain]s (aa 29-92, aa 102-164, aa 181-243, aa 258-320) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ]
  description: [Pubmed|9383190]

Biological materials

Mutant

• BKE27580 (Δ[gene|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTCCTCCTTTCTGA, downstream forward: _UP4_TAAAAAAAGCTGCCTTTTGG
• BKK27580 (Δ[gene|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTCCTCCTTTCTGA, downstream forward: _UP4_TAAAAAAAGCTGCCTTTTGG

References

• 9383190