pbpG [2019-06-25 11:32:02]

penicillin-binding protein 2d

locus: BSU37510

pI: 9.39

mw: 71.66 kDa

protein length: 691 aa

gene length: 2076 bp

function: bifunctional glucosyltransferase/ transpeptidase

product: penicillin-binding protein 2d

essential: no

synonyms: ywhE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,849,818 → 3,851,893

Phenotypes of a mutant

• a strain lacking all four class A [SW|penicillin-binding proteins] ([gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|pbpD] [gene|73275060537497E6A9859856C9F040763E36316B|pbpF] [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]

The protein

Paralogous protein(s)

• [protein|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|PbpD], [protein|73275060537497E6A9859856C9F040763E36316B|PbpF], [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PonA]

Structure

• [PDB|3VMA] (PBP1B from E. coli, 29% identity) [pubmed|19458048]

[SW|Localization]

• cell membrane, the major part of the protein is exposed to the outside (according to UniProt)
• prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]

Expression and Regulation

Operons

• genes: [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325,15758244,10767540], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigF], [protein|search|SigG]) [Pubmed|16497325,15758244,10767540]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|pbpG]' and '[protein|search|ywhD]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-A520 (ywhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/520 NBRP B. subtilis, Japan]
• BKE37510 (Δ[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTC
• BKK37510 (Δ[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTC

GFP fusion

• 3510 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::pSG5309 (cat P_xyl-gfpa-[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]1-544) [pubmed|15758244], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

References

• 16497325,11567005,10767540,20525796,15758244,19458048,29456081