SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

ydhE

similar to macrolide glycosyltransferase

locus: BSU_05720

pI: 5.09

mw: 42.87 kDa

protein length: 395 aa

gene length: 1188 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]

Gene

Coordinates: 618,095 → 619,282

The protein

Protein family

• UDP-glycosyltransferase family (with [protein|02927D2CC9F44B99DB307DDFED2DD52DE2196120|YjiC] and [protein|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|YojK], according to UniProt)

Structure

• [PDB|2IYA] (from Streptomyces antibioticus, 28% identity) [pubmed|17376874]

Expression and Regulation

Operons

• genes: [gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]
  description: [Pubmed|20639339]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, weak, in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]

Biological materials

Mutant

• MGNA-C180 (ydhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2178 NBRP B. subtilis, Japan]
• BKE05720 (Δ[gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAGACAACTCCCTCTC, downstream forward: _UP4_TAAGAAAAAGAACTCCCGTA
• BKK05720 (Δ[gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAGACAACTCCCTCTC, downstream forward: _UP4_TAAGAAAAAGAACTCCCGTA

References

• 20639339,27130432,17376874,31786106