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rpoC [2020-03-12 17:13:06]
[SW|RNA polymerase] beta' subunit
function
[SW|transcription]
product
[SW|RNA polymerase] beta' subunit
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
125,562 → 129,161
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)Protein family
[SW|RNA polymerase] beta' chain family (single member, according to UniProt)Modification
phosphorylated on Arg-335 and Arg-803 [Pubmed|22517742]Structure
[PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 50% identity) [Pubmed|19903881][SW|Localization]
colocalizes with the nucleoid [Pubmed|25092033]membrane associated [Pubmed|18763711]Expression and Regulation
Operons
genes
[gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7657605], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]view in new tabBiological materials
Expression vector
pGP2180: expression of ''rpoC''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s labtwo-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s labReferences
Reviews
22210308,24763425,28657884 Original publications
7657605,12486038,10675340,7592585,19735077,18763711,19680289,22517742,23070162,20817769,24572018,25092033,24491578,26243774,21357417,26293966,26742846,19903881,17917675,26098117,28507241,28539362,28598017