SubtiBank SubtiBank
glcT [Tue Jul 05 2016 18:02:36 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

glcT [Tue Jul 05 2016 18:02:36 GMT+0200 (CEST)]

transcriptional antiterminator , controls expression of the ptsG-ptsH-ptsI operon
locus
BSU13880
pI
7.00
mw
33.00 kDa
function
control of glucose uptake
product
transcriptional antiterminator of the ptsG-ptsH-ptsI operon
essential
no
synonyms
ykwA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,456,092 -> 1,456,958

    The protein

    Catalyzed reaction/ biological activity

  • [PRD-containing transcription factors|search|transcription antiterminator] , RNA-binding protein, binds the ''[protein|search|ptsG]'' RAT sequence
  • Protein family

  • [PRD-containing transcription factors|search|transcription antiterminator] of the BglG/ [protein|search|SacY] family
  • Paralogous protein(s)

  • [protein|search|LicT], [protein|search|SacT], [protein|search|SacY]
  • [SW|Domains]

  • RNA-binding domain (N-terminal, constitutive antiterminator)
  • 2x [SW|PTS] regulation domains (PRDs) (C-terminal, neg. regulated by [protein|search|PtsG])
  • Modification

  • phosphorylation (His104)
  • Structure

  • [PDB|3GWH] (PRD-II) [Pubmed|19684596]
  • [PDB|3RIO] (RBD-PRD-I) [Pubmed|22750856]
  • [SW|Interactions]

  • [protein|search|PtsH]-[protein|search|GlcT]
  • Biological materials

    Mutant

  • available in [SW|Jörg Stülke]'s lab:
  • GP109 (in frame deletion)
  • GP777 (replacement of ''[protein|search|glcT]'' and the ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' operon by a spc cassette), [Pubmed|22722928]
  • GP926 (substitution of ''[protein|search|glcT]'' and ''[protein|search|ptsG]'' by a tet cassette), [Pubmed|22722928]
  • Expression vector

  • pGP124 (full length, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP114 (amino acids 1-60, RNA-binding domain, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP164 (both PRDs, in [SW|pWH844]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP424 (PRDI, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP425 (PRDII, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP442 (PRDI, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
  • pGP443 (PRDII, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
  • pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1224 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1220 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • References

    Reviews

  • 9663674,18086213
  • Original publications

  • 11902727,9765562,12437213,10543968,17074746,15155854,14527945,19684596,22722928,20939030,22750856