nrnB

oligoribonuclease (nano-RNase), degradation of pGpG

Locus: BSU_18200

Isoelectric point: 5.03

Molecular weight: 46.28 kDa

Protein length: 399 aa

Gene length: 1200 bp

Function: degradation of RNA oligonucleotides

Product: nano-RNase B

Essential: no

Synonyms: yngD

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
• Lifestyles → Sporulation → Sporulation proteins → Newly identified sporulation proteins (based on transcription profiling)

Gene

Coordinates: 1,948,313 → 1,949,512

The protein

Catalyzed reaction/ biological activity

• degradation of RNA oligonucleotides PubMed
• degradation of pGpG derived from c-di-GMP PubMed

Protein family

• DHH/DHHA1 protein family of phosphoesterases PubMed

Expression and Regulation

Operons

• Genes: nrnB
  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B398 (yngD::erm), available at the NBRP B. subtilis, Japan
• BKE18200 (ΔnrnB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG
• BKK18200 (ΔnrnB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG

References

Reviews

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Original publications

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