glpD [Mon Aug 31 2015 13:53:20 GMT+0200 (CEST)]

glycerol-3-phosphate dehydrogenase (menaquinone 7)

locus: BSU09300

pI: 8.00

mw: 62.00 kDa

function: glycerol utilization

product: glycerol-3-phosphate dehydrogenase (menaquinone 7)

essential: no

ec: 1.1.5.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.3|Utilization of glycerol/ glycerol-3-phosphate]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.1|Utilization of phospholipids]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources], [SW|utilization of lipids]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon], [SW|CcpA regulon], [SW|GlpP regulon]

The protein

Catalyzed reaction/ biological activity

• sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)

Protein family

• FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

[SW|Cofactors]

• FAD

Structure

• [PDB|3DA1] (from ''Bacillus halodurans'', 57% identity, 72% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|glpD]'' [Pubmed|2127799]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|1809833]

Regulation

• repressed by glucose ([protein|search|CcpA])
• induced by glycerol ([protein|search|GlpP]) [Pubmed|1809833]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression
• [protein|search|GlpP]: transcriptional antitermination at a [SW|RNA switch] [Pubmed|1809833]
• [protein|search|AbrB]: transcription activation [Pubmed|20817675]

Biological materials

Mutant

• QB5347 (cat), available in the [SW|Stülke] lab

References

• 8825777,1479885,9595668,9493382,20817675