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glyA [Wed Dec 21 2016 14:51:09 GMT+0100 (CET)]
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glyA [Wed Dec 21 2016 14:51:09 GMT+0100 (CET)]

serine hydroxymethyltransferase
locus
BSU36900
pI
5.00
mw
45.00 kDa
function
biosynthesis of glycine
product
serine hydroxymethyltransferase
essential
yes
ec
2.1.2.1
synonyms
glyC, ipc-34d

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|essential genes], [SW|phosphoproteins], [SW|most abundant proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|PurR regulon], [SW|T-box]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,789,190 -> 3,790,437

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • inactivation of ''[SW|glyA]'' suppresses the synthetic lethality of the ''[SW|defA] [SW|defB]'' double mutant [Pubmed|27983482]
  • The protein

    Catalyzed reaction/ biological activity

  • 5,10-methylenetetrahydrofolate glycine H2O = tetrahydrofolate L-serine (according to Swiss-Prot)
  • Protein family

  • SHMT family (according to Swiss-Prot)
  • Modification

  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]
  • Structure

  • [PDB|2VGU] (complex with L-serine, Geobacillus stearothermophilus), [PDB|2VI8] (Geobacillus stearothermophilus)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operon

  • ''[protein|search|glyA]'' (according to [http://dbtbs.hgc.jp/COG/prom/glyA.html DBTBS])
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11591660]
  • Regulation

  • repressed in the presence of adenine or adenosine([protein|search|PurR]) [Pubmed|2536750]
  • induced by glycine limitation ([protein|search|T-box]) [Pubmed|19258532]
  • Regulatory mechanism

  • [protein|search|T-box]: [SW|RNA switch], transcriptional antitermination [Pubmed|19258532]
  • [protein|search|PurR]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
  • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • References

  • 19258532,11591660,20152942,19171795,17726680,16493705,15378759,27983482