glnH

glutamine [SW|ABC transporter] (binding protein)

locus: BSU_27440

pI: 8.55

mw: 29.60 kDa

protein length: 273 aa

gene length: 822 bp

function: glutamine uptake

product: glutamine [SW|ABC transporter] (binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,803,108 → 2,803,929

The protein

Protein family

• [SW|bacterial solute-binding protein 3 family] (according to UniProt)

Structure

• [PDB|2V25] (from ''Campylobacter jejuni'', 37% identity) [Pubmed|17631313]

[SW|Localization]

• attached to the membrane via [protein|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|GlnM]-[protein|CD32011A08697B2C121996A02F1BD7BE4F932778|GlnP] [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ]-[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]-[gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]-[gene|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]
  description: [Pubmed|12823818]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|15699190]

Biological materials

Mutant

• GP734 (spc), available in the [SW|Stülke] lab
• BKE27440 (Δ[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA
• BKK27440 (Δ[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA

References

• 10092453,12823818,25755103,15699190,17631313,20023025