RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
function
cleaves both 5- and 3-sites
of the small cytoplasmic RNA precursor (scr)
product
endoribonuclease III
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|RNases], [SW|translation], [SW|protein secretion], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|stringent response]Gene
Coordinates on the chromosome (coding sequence)
1,665,710 -> 1,666,459
Phenotypes of a mutant
essential [Pubmed|23300471,12682299,11123676]the ''[protein|search|rnc]'' gene can be deleted in strains cured of the [SW|Skin element] and [[SPß prophage]] (or upon deletion of the toxin genes ''[protein|search|txpA]'' and ''[protein|search|yonT]'') [Pubmed|23300471]The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[protein|search|scr]'')degradation of ''[protein|search|txpA]'' mRNA-[protein|search|RatA] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]degradation of ''[protein|search|yonT]'' mRNA-''[protein|search|as-yonT]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]degradation of the ''[protein|search|roxS]''-''[SW|ppnKB]'' and ''[protein|search|roxS]-[protein|search|sucC]'' RNA duplexes [Pubmed|25643072]Structure
[PDB|1O0W] (from ''Thermotoga maritima'', 41% identity)[SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operon
''[protein|search|rnc]-[protein|search|smc]-[protein|search|ftsY]'' [Pubmed|11021934][SW|Sigma factor]
[protein|search|SigA] [Pubmed|11021934]Regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] labLabs working on this gene/protein
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage][SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]References
Reviews
17194582,16179796,8936814,16855311,23300472,25292357,25808661,25878039 Original publications
9393702,11123676,7527387,9677377,17576666,11021934,6409421,22123973,22412379,25643072,23300471,26940229,26883633 
