SubtiBank SubtiBank
yumC
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

yumC

ferredoxin/flavodoxin-NAD(P) oxidoreductase
locus
BSU32110
pI
5.57
mw
36.68 kDa
protein length
332 aa Sequence Blast
gene length
996 bp Sequence Blast
function
redox reactions that involve ferredoxin and flavodoxin
product
ferredoxin/flavodoxin reductase
essential
yes
ec
1.18.1.2
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,301,586 → 3,302,584

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2 reduced ferredoxin or flavodoxin NADP H = 2 oxidized ferredoxin or flavodoxin NADPH (according to Swiss-Prot)
  • Protein family

  • ferredoxin-NADP reductase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|YcgT]
  • Modification

  • active site Cys85 is S-bacillithiolated by NaOCl stress in ''B. subtilis'' and other ''Bacillus'' species [http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed]
  • [SW|Cofactors]

  • FAD [Pubmed|20878669]
  • Structure

  • [PDB|3LZW] [Pubmed|20878669]
  • Expression and Regulation

    Operons

    genes
    [gene|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC]
    description
    [Pubmed|22383849]
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    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
  • Biological materials

    Mutant

  • MGNA-B572 (yumC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1571 NBRP B. subtilis, Japan]
  • References

  • 22938038,20208166,20878669,19162251,15252706,24529496,25194416,25698107,26507228,26965753,26883633