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ybfO
similar to erythromycin esterase
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]Gene
Coordinates
249,979 → 251,319
The protein
Structure
[PDB|2QGM] (23% identity)[SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]Expression and Regulation
Operons
genes
[gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]-[gene|1EE8BF589ECDFD5194C86E02A627CCF81899B904|ybfP]-[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]
description
[Pubmed|12207695]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696,20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]) [Pubmed|18840696]view in new tabgenes
[gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]-[gene|1EE8BF589ECDFD5194C86E02A627CCF81899B904|ybfP]
description
[Pubmed|20817675]
regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]) [Pubmed|18840696]view in new tabgenes
[gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]
description
(according to [http://dbtbs.hgc.jp/COG/prom/ybfO.html DBTBS]) null
regulation
repressed during logarithmic growth ([protein|search|AbrB]) [Pubmed|18840696]additional information
[protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]view in new tabadditional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]Biological materials
Mutant
MGNA-B935 (ybfO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1934 NBRP B. subtilis, Japan]BKE02310 (Δ[gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCTATCTCTCCTTTTC, downstream forward: _UP4_TGAGCGGTGTCCCCTGTGGTBKK02310 (Δ[gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCTATCTCTCCTTTTC, downstream forward: _UP4_TGAGCGGTGTCCCCTGTGGTReferences
18957862,11866510,18840696,12207695,20817675