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rlmCD [2018-01-03 17:01:34]

rRNA methyltransferase

locus

BSU06730

5.71

51.65 kDa

protein length

459 aa Sequence Blast

gene length

1377 bp Sequence Blast

function

23S rRNA maturation

product

rRNA methyltransferase

essential

2.1.1.190

synonyms

yerS, yefA

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates

737,603 → 738,982

The protein

Catalyzed reaction/ biological activity

methylation of U747 and U1939 in 23S rRNA [Pubmed|21824914]

Protein family

COG2265

Paralogous protein(s)

[protein|05392BB2C32B0405799D4F34E5BA55FC936AB943|YfjO]

Structure

[PDB|2BH2] (from ''E. coli'', 32% identity) [Pubmed|15766524]

Expression and Regulation

Operons

genes

[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]

description

[Pubmed|22383849]

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genes

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-A931 (yefA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/931 NBRP B. subtilis, Japan]

BKE06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA

BKK06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA

References

21824914,15766524