hemAT [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

soluble chemotaxis receptor, heme-containing O(2) sensor protein

locus: BSU10380

pI: 5.44

mw: 48.00 kDa

function: movement towards oxygen

product: haem-based aerotactic transducer

essential: no

synonyms: yhfV

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.3|Soluble chemoreceptors]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]

[SW|Categories] containing this gene/protein

• [SW|motility and chemotaxis]

This gene is a member of the following [SW|regulons]

• [SW|SigD regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,112,620 -> 1,113,918

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• NCIB3610 ''hemAT'' mutant has higher fitness than ''[protein|search|hag]'' mutant during pellicle formation [Pubmed|26122431]
• NCIB3610 ''hemAT''-''[protein|search|hag]'' double mutant has similar fitness to single ''[protein|search|hag]'' mutant during pellicle formation [Pubmed|26122431]

The protein

Catalyzed reaction/ biological activity

• required for full expression of the ''[protein|search|phoD]-[SW|tatAD]-[SW|tatCD]'' operon [Pubmed|23180473]

[SW|Cofactors]

• heme

Structure

• [PDB|1OR4] (in cyano-liganded form), [PDB|1OR6] [Pubmed|12962628]

[SW|Localization]

• forms clusters at the cell poles [Pubmed|21515776]
• homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase [Pubmed|23180473]
• polar foci in individual cells at later growth stages [Pubmed|23180473]

[SW|Interactions]

• [SW|HemAT]-[SW|TatAC] [Pubmed|23180473]

Expression and Regulation

Operon

• ''hemAT'' [Pubmed|15033535]

[SW|Sigma factor]

• [protein|search|SigD] [Pubmed|15033535]

Additional information

• in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• TB239 (''hemAT''::''neo'' in 168) [Pubmed|26122431]
• TB241 (''hemAT''::''neo'' in NCIB3610) [Pubmed|26122431]

Labs working on this gene/protein

• [SW|Akos T Kovacs]

References

Reviews

• 23928310

Original publications

• 26122431,10676961,16819829,11481493,15033535,21515776,23180473,22564695