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splB [Mon Jan 02 2017 11:42:55 GMT+0100 (CET)]
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splB [Mon Jan 02 2017 11:42:55 GMT+0100 (CET)]

spore photoproduct lyase
locus
BSU13930
pI
8.00
mw
39.00 kDa
function
protection of spore DNA against photodamage
product
spore photoproduct lyase
essential
no
synonyms
spl

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigG regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,461,770 -> 1,462,798

    The protein

    Catalyzed reaction/ biological activity

  • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
  • [SW|Cofactors]

  • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
  • Structure

  • [PDB|4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) [Pubmed|22761404]
  • Expression and Regulation

    Operon

  • ''[protein|search|splA]-[protein|search|splB]'' [Pubmed|8021181]
  • [SW|Sigma factor]

  • [protein|search|SigG] [Pubmed|15699190,8021181]
  • Regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]
  • Regulatory mechanism

  • negative autoregulation by [protein|search|SplA] [Pubmed|8021181]
  • Biological materials

    Mutant

  • BP130 (del ''splB''::''spc''), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 23164663,25477522
  • Structure of the spore photoproduct lesion in DNA

  • 24598744
  • Original publications

  • 15699190,8021181,21671623,22197590,23607538,23799365,22761404,28027411