spore photoproduct lyase, radical SAM enzyme
function
protection of spore DNA against photodamage
product
spore photoproduct lyase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|sporulation proteins]This gene is a member of the following [SW|regulons]
[SW|SigG regulon]Gene
Coordinates on the chromosome (coding sequence)
1,461,770 -> 1,462,798
The protein
Catalyzed reaction/ biological activity
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase[SW|Cofactors]
contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAMStructure
[PDB|4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) [Pubmed|22761404]Expression and Regulation
Operon
''[protein|search|splA]-[protein|search|splB]'' [Pubmed|8021181][SW|Sigma factor]
[protein|search|SigG] [Pubmed|15699190,8021181]Regulation
expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]Regulatory mechanism
negative autoregulation by [protein|search|SplA] [Pubmed|8021181]Biological materials
Mutant
BP130 (del ''splB''::''spc''), available in [SW|Fabian Commichau]'s labReferences
Reviews
23164663,25477522,22197590 Structure of the spore photoproduct lesion in DNA
Original publications
15699190,8021181,21671623,22197590,23607538,23799365,22761404,28027411,16829676,11470912,22906093 
