yxjF

sporulation protein, 3-hydroxybutyrate dehydrogenase

locus: BSU_38970

pI: 7.89

mw: 27.46 kDa

protein length: 257 aa

gene length: 774 bp

function: 3-hydroxybutyrate utilization

product: 3-hydroxybutyrate dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,000,539 → 4,001,312

The protein

Protein family

• [SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Paralogous protein(s)

• [protein|D945CAD7209CB94A7B9E23263476124B7E8853DD|YusZ]
• [protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|YxbG]:

Structure

• [PDB|2YZ7] (from Alcaligenes Faecalis 42% identity)

Expression and Regulation

Operons

• genes: [gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135,10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]

Biological materials

Mutant

• MGNA-B730 (yxjF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1729 NBRP B. subtilis, Japan]
• GP1112 (spc), available in [SW|Stülke] lab
• BKE38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG
• BKK38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG

References

• 16672620,10666464,12850135,10746760,12662922