SubtiBank SubtiBank
yfkN [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yfkN [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

cell wall-anchored 3'5' cyclic dinucleotide 3' phosphodiesterase, degrades c-di-AMP
locus
BSU07840
pI
5.00
mw
159.00 kDa
function
degradation of c-di-AMP
product
cell wall-anchored 3'5' cyclic dinucleotide 3′ phosphodiesterase, degrades c-di-AMP
essential
yes
ec
3.1.4.16
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides], [SW|essential genes], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|PhoP regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    855,114 -> 859,502

    Phenotypes of a mutant

  • essential [Pubmed|17114254]
  • The protein

    Catalyzed reaction/ biological activity

  • degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]
  • Paralogous protein(s)

  • [protein|search|YhcR]
  • [SW|Cofactors]

  • Mn2 [Pubmed|27414497]
  • Structure

  • [PDB|3GVE]
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862], the transmembrane domain serves as retention signal, attached to the cell wall [Pubmed|21800427,22767609]
  • [SW|Interactions]

  • [protein|search|YhcS]-[protein|search|YfkN] [Pubmed|21800427] (anchoring to the cell wall)
  • Expression and Regulation

    Operon

  • ''yfkN'' [Pubmed|16291680]
  • Regulation

  • repressed by glucose (5.2-fold) ([protein|search|CcpA]) [Pubmed|12850135]
  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]
  • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|12850135]
  • [protein|search|PhoP]: transcription activation [Pubmed|16291680]
  • Biological materials

    Mutant

  • MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]
  • References

  • 14688230,16291680,18957862,12850135,17114254,21800427,27414497