SubtiBank SubtiBank
pepT
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pepT

peptidase T (tripeptidase), zinc-dependent
locus
BSU_38920
pI
4.54
mw
45.35 kDa
protein length
410 aa Sequence Blast
gene length
1233 bp Sequence Blast
function
peptide degradation
product
peptidase T (tripeptidase)
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
  • Gene

    Coordinates
    3,995,075 → 3,996,307

    The protein

    Catalyzed reaction/ biological activity

  • Release of the N-terminal residue from a tripeptide (according to UniProt)
  • Protein family

  • peptidase M20B family (single member, according to UniProt)
  • Paralogous protein(s)

  • [protein|4D56C583BBAF47F384ACFACF7A607FD8BFA59960|YqjE]
  • Structure

  • [PDB|3GBO] (from ''Bacillus cereus (71% identity''), to be published)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]
    description
    [Pubmed|10746760]
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    Biological materials

    Mutant

  • MGNA-B733 (pepT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1732 NBRP B. subtilis, Japan]
  • BKE38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT, downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA
  • BKK38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT, downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA
  • References

  • 8978088,10987140