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dfrA

dihydrofolate reductase

locus: BSU_21810

pI: 5.22

mw: 19.03 kDa

protein length: 168 aa

gene length: 507 bp

function: biosynthesis of folate

product: dihydrofolate reductase

essential: yes

ec: 1.5.1.3

synonyms: tmp

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,296,603 → 2,297,109

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ --> 7,8-dihydrofolate + H⁺ + NADPH (according to UniProt)

Protein family

• dihydrofolate reductase family (single member, according to UniProt)

[SW|Domains]

• DHFR domain (aa 1-159) (according to UniProt)

Structure

• [PDB|1ZDR] (Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]-[gene|2CB3BAF17F59A858D249619A436EF660CAA58DD5|ypjQ]-[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]-[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]
  description: [Pubmed|22383849]

• genes: [gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
  description: [Pubmed|2840350]

  regulatory mechanism

  • [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN]: activation, weak, in [regulon|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN regulon]

  regulation

  • weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]

References

• 2840350,6436144,3918568,24158440