SubtiBank SubtiBank
dfrA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

dfrA

dihydrofolate reductase
locus
BSU21810
pI
5.22
mw
19.03 kDa
protein length
168 aa Sequence Blast
gene length
504 bp Sequence Blast
function
biosynthesis of folate
product
dihydrofolate reductase
essential
yes
ec
1.5.1.3
synonyms
tmp

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,296,603 → 2,297,109

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)
  • Protein family

  • spmB family (according to Swiss-Prot)
  • Structure

  • [PDB|1ZDR] (Geobacillus stearothermophilus)
  • Expression and Regulation

    Operons

    genes
    [gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
    description
    [Pubmed|2840350]

    regulatory mechanism

  • [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN]: activation, weak, in [regulon|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN regulon]
  • regulation

  • weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]
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    References

  • 2840350,6436144,3918568,24158440