SubtiBank SubtiBank
pucD
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pucD

xanthine dehydrogenase
locus
BSU32480
pI
5.75
mw
80.25 kDa
protein length
745 aa Sequence Blast
gene length
2238 bp Sequence Blast
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
yurC

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
  • Gene

    Coordinates
    3,336,263 → 3,338,500

    The protein

    Catalyzed reaction/ biological activity

  • Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Protein family

  • xanthine dehydrogenase family (according to Swiss-Prot)
  • Structure

  • [PDB|1RM6] (4-hydroxybenzoyl-CoA reductase from Thauera aromatica, 30% identity) [pubmed|15576037]
  • Expression and Regulation

    Operons

    genes
    [gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • regulation

  • induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
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    Biological materials

    Mutant

  • MGNA-A964 (yurC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/964 NBRP B. subtilis, Japan]
  • BKE32480 (Δ[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC
  • BKK32480 (Δ[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC
  • References

  • 11344136,12029039,12823818,15576037