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mutY
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mutY

A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches
locus
BSU_08630
pI
6.04
mw
41.81 kDa
protein length
369 aa Sequence Blast
gene length
1110 bp Sequence Blast
function
DNA repair
product
A/G-specific adenine glycosylase
essential
no
ec
3.2.2.-
synonyms
yfhQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.5|Oxidized guanine (GO) DNA repair system]

    • Gene

    Coordinates
    935,656 → 936,765

    Phenotypes of a mutant

  • strongly reduced stationary phase mutagenesis [Pubmed|27399782]
  • increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]

    • The protein

    Catalyzed reaction/ biological activity

  • releases adenines from 8-oxo-G:A mismatches
  • Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site (according to UniProt)

    • Protein family

  • Nth/MutY family (with [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth], according to UniProt)

    • [SW|Domains]

  • HhH domain (aa 108-137) (according to UniProt)

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|1RRS] (complex with DNA, Geobacillus stearothermophilus)

    • Expression and Regulation

    Operons

    genes
    [gene|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]-[gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]-[gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]
    description
    [Pubmed|10463184]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10463184], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1900507], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP]: repression, in [regulon|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP regulon]

    • regulation

  • expression is constitutive throughout growth [Pubmed|20971907]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C322 (yfhQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2320 NBRP B. subtilis, Japan]
  • BKE08630 (Δ[gene|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC
  • BKK08630 (Δ[gene|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT, downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC

    • References

    Reviews

  • 22933559

    • Original publications

  • 10463184,19011023,20971907,24973075,25326311,25995449,27399782,30691388