SubtiBank SubtiBank
putP
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putP

high affinity proline permease
Locus
BSU_03220
Isoelectric point
8.62
Molecular weight
48.25 kDa
Protein length
473 aa Sequence Blast
Gene length
1422 bp Sequence Blast
Function
proline uptake
Product
high affinity proline permease
Essential
no
Synonyms
ycgO

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
347,150 → 348,571

Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • uptake of proline coupled to the uptake of sodium ions
  • Protein family

    Paralogous protein(s)

    Structure

  • 2XQ2 (from Vibrio parahaemolyticus, 26% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Genes
    Description
    (internal promoter) PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Additional information

  • the mRNA is cleaved by RNase III PubMed
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    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • PutR: activation, PubMed, in PutR regulon
  • Spo0A: activation, PubMed, in Spo0A regulon
  • CodY: repression, displacement of PutR PubMed, in CodY regulon
  • Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • Additional information

  • overexpressed in a pnpA mutant PubMed
  • the putP part of the mRNA is cleaved by RNase III PubMed
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    Biological materials

    Mutant

  • MGNA-B994 (ycgO::erm), available at the NBRP B. subtilis, Japan
  • available in Erhard Bremer's lab PubMed
  • BKE03220 (ΔputP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCGCCATATAAATAC, downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
  • BKK03220 (ΔputP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCGCCATATAAATAC, downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
  • References

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