yqfL

Modulator of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity, similar to phosphoenolpyruvate synthetase regulatory protein

locus: BSU_25240

pI: 5.78

mw: 30.20 kDa

protein length: 270 aa

gene length: 813 bp

function: inhibits [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity

product: modulator of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity

essential: no

ec: 2.7.-.-

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

Gene

Coordinates: 2,604,121 → 2,604,933

The protein

Catalyzed reaction/ biological activity

• negative effector of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity [Pubmed|15720552]
• ADP + N^τ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H⁺ + N^τ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)
• H⁺ + N^τ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + N^τ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)

Protein family

• pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)

[SW|Domains]

• nucleotide binding domain (ATP) (151–158)

Structure

• [PDB|5D0N] (from maize, 39% identity) [pubmed|26620526]

Expression and Regulation

Operons

• genes: [gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]-[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]
  description: [Pubmed|15720552]

  regulation

  • constitutive [Pubmed|15720552]

  additional information

  • the intracellular concentration of CcpN is about 4 myM (according to [PubMed|20408793]).

Biological materials

Mutant

• MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]
• BKE25240 (Δ[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC
• BKK25240 (Δ[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCC

labs

• [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 15720552,26620526,20044937