efeO

lipoprotein, elemental iron uptake system (binding protein), high affinity uptake of ferric iron (Fe(III))

locus: BSU_38270

pI: 4.71

mw: 42.63 kDa

protein length: 385 aa

gene length: 1158 bp

function: elemental iron uptake

product: elemental iron uptake system (binding protein)

essential: no

synonyms: ywbM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.2|Elemental iron transport system]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.5|Elemental iron transport system]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,927,951 → 3,929,108

The protein

Catalyzed reaction/ biological activity

• binds ferric iron (Fe(III)) with strong selectivity [Pubmed|23764491]

Protein family

• EfeM/EfeO family (single member, according to UniProt)

Structure

• [PDB|3AT7] (from ''Sphingomonas sp.'', 40% identity) [Pubmed|21238429]

[SW|Localization]

• membrane [Pubmed|18763711]
• extracytosolic lipoprotein [Pubmed|23764491]

Expression and Regulation

Operons

• genes: [gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23764491], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9683469], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]

Biological materials

Mutant

• MGNA-B227 (ywbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1226 NBRP B. subtilis, Japan]
• BKE38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA, downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA
• BKK38270 (Δ[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGATACAGCGATTTTTGTGA, downstream forward: _UP4_CTATAAAAGGAGTTTTGTAA

References

• 16672620,9353933,12354229,18763711,23180473,23764491,21238429,27795321,29133393