SubtiBank SubtiBank
kinA [Fri May 27 2016 15:40:10 GMT+0200 (CEST)]
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kinA [Fri May 27 2016 15:40:10 GMT+0200 (CEST)]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
68.00 kDa
initiation of sporulation
two-component sensor kinase
spoIIF, spoIIJ, gsiC, scoD

Genomic Context



  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW|Control of two-component response regulators] → [category|SW|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW|The kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigH regulon], [SW|Spo0A regulon], [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,470,026 -> 1,471,846

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [SW|Spo0F]
  • mainly active in the older, inner regions of a colony (with [protein|search|KinB]) [Pubmed|21097618]
  • [SW|Domains]

  • three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
  • the first [SW|PAS domain] is required for NAD( ) binding [Pubmed|23599347]
  • C-terminal histidine phosphotransferase domain
  • Modification

  • autophosphorylation on a His residue
  • Effectors of protein activity

  • interaction with [protein|search|KipI] or [protein|search|Sda] inhibits autophosphorylation of [protein|search|KinA] [Pubmed|9334321,15023339]
  • autophosphorylation is inhibited by [protein|search|SivA] and [protein|search|BslA] [Pubmed|23335417]
  • activated by interaction with NAD( ) that indicated poor respiratory activity of the cell [Pubmed|23599347]
  • Structure

  • [PDB|2VLG] (PAS domain)
  • [SW|Localization]

  • cytoplasm
  • [SW|Interactions]

  • forms tetramers [Pubmed|21926229]
  • [protein|search|KinA]-[protein|search|KipI] [Pubmed|9334321]
  • [protein|search|KinA]-[protein|search|Sda] [Pubmed|15023339]
  • [SW|Spo0F]-[protein|search|KinA]
  • Expression and Regulation


  • ''[protein|search|kinA]'' [Pubmed|1569009]
  • [SW|Sigma factor]

  • [protein|search|SigH] [Pubmed|1569009]
  • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|9644251]
  • induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|9644251]
  • Additional information

  • promoter-up mutations or high artificial expression that render ''[SW|kinA]'' independent from [SW|SigH], allow [SW|sporulation] at high salinity [Pubmed|26712348]
  • References


  • 22303284
  • Original Publications

  • 23169620,20511506,19561131,20413551,1846779,11292798,16166384,11734624,9299348,12067336,18174125,10094672,11069677,15023339,2509430,17350039,9334321,9477965,9644251,1569009,20081035,21097618,21926229,22303282,22670053,23335417,23378509,23504013,23599347,25598361,26055117,26165942,26657919,26712348