prp

protein cysteine protease, required for N-terminal cleavage of [protein|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|ribosomal protein L27]

locus: BSU_27950

pI: 4.46

mw: 12.16 kDa

protein length: 112 aa

gene length: 339 bp

function: maturation of [protein|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|ribosomal protein L27]

product: [protein|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|ribosomal protein L27] cysteine protease

essential: yes

synonyms: ysxB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,855,177 → 2,855,515

Phenotypes of a mutant

• essential [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• N-terminal cleavage of [protein|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|ribosomal protein L27] [Pubmed|25388641]

Structure

• [PDB|2P92] (the protein from ''S. aureus'')

Expression and Regulation

Operons

• genes: [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • likely autorepression of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] operon expression upon binding of excess of one ribosomal protein to the untranslated region of the [gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]→[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA] mRNA [Pubmed|17616982]

• genes: [gene|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA]-[gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]-[gene|11927EB0448839D047EC7BF70EC65156DAB481D3|rplU]-[gene|B27548A4BBF396C9BA102A80ACB08B21262CEA30|prp]-[gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|rpmA]
  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]

  additional information

  • the domains that inhibits [protein|search|SpoIVFB] is cleaved off by [protein|search|CtpB] (second cleavage) [PubMed|24243021]

References

• 11948165,25388641,22383849,28187498,28189581